Effect of Buyang Huanwu Tang on Synaptic Structural Plasticity After Cerebral Ischemia-reperfusion in Rats
10.13422/j.cnki.syfjx.20192201
- VernacularTitle:补阳还五汤对脑缺血再灌注大鼠恢复期突触可塑性的影响
- Author:
Kai-ling YANG
1
;
Ying ZHOU
1
;
Fu-man YAN
1
;
Le-quan ZHOU
1
;
Yu-lian LIU
1
;
Wei LIU
1
Author Information
1. School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
- Publication Type:Research Article
- Keywords:
Buyang Huanwu Tang;
cerebral ischemia;
connexin43 (Cx43);
synaptic structural plasticity;
synaptophysin (SYN);
growth associated protein-43 (GAP-43)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(1):43-49
- CountryChina
- Language:Chinese
-
Abstract:
Objective::To investigate the mechanism of Buyang Huanwu Tang (BYHWT) in improving synaptic structural plasticity after cerebral ischemia-reperfusion in rats. Method::Middle cerebral artery occlusion and reperfusion model was established. SD rats were randomly divided into sham-operated group, model group, BYHWT group, BYHWT+ Gap26(connexin43 inhibitor)groups. BYHWT was given twice a day(16 g·kg-1), Gap26 was intraperitoneally injected once a day since the third day after surgery (25 g·kg-1). Brain was taken out at the 7th day. The changes of neuronal synaptic and gap junction ultrastructure were observed by transmission electron microscopy. Synaptophysin (SYN) and growth-associated protein-43 (GAP-43) protein expression were detected by Western blot and immunofluorescence. Result::The structure of synapses was integrated, and the gap junctions were clear in sham-operated group. In the hippocampus of model group, the structure was destroyed, and the gap junctions disappeared. Compared with the sham-operated group, model group up-regulated the expressions of SYN and GAP-43 (P<0.05, P<0.01). In the hippocampus of BYHWT group, the structure was close to the normal. Furthermore, BYHWT up-regulated the expressions of SYN and GAP-43 (P<0.05, P<0.01). However, after the combined administration with Cx43 inhibitor (Gap26), the damage of synaptic structural decreased, only a small number of gap junctions with the structural integrity can be seen, and the effect of BYHWT on SYN and GAP-43 was inhibited (P<0.05, P<0.01). Conclusion::BYHWT could improve the hippocampal synaptic structural plasticity obviously after the CIRI. The mechanism may be related to the increase of the expression of Cx43 and the promotion of the intervention of SYN and GAP-43.
