Analysis and Identification of Chemical Constituents of Citri Sarcodactylis Fructus by UPLC-Q-Orbitrap HRMS
10.13422/j.cnki.syfjx.20200715
- VernacularTitle:基于UPLC-Q-Orbitrap HRMS的川佛手化学成分分析
- Author:
Han-wen HU
1
;
Yong-yan ZHAO
1
;
Tian-long YANG
1
;
Zhen-xing ZHENG
1
;
Teng PENG
1
;
Fang DENG
1
Author Information
1. Key Laboratory of Standardization of Chinese Herbal Medicine under Ministry of Education, State Key Laboratory and Breeding Base for Systematic Research Development and Utilization of Chinese Medical Resources, Co-founded by Sichuan Province and Ministy of Science and Technology, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
- Publication Type:Research Article
- Keywords:
Citri Sarcodactylis Fructus;
chemical constituents;
UPLC-Q-Orbitrap HRMS;
flavonoids;
coumarins;
limonoids
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(7):148-155
- CountryChina
- Language:Chinese
-
Abstract:
Objective::To analyze and identify the chemical constituents of Citri Sarcodactylis Fructus by an ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap high-resolution mass spectrometry (UPLC-Q-Orbirap HRMS) method. Method::Compounds were separated on Thermo Scientific Accucore™ C18 column (3 mm×100 mm, 2.6 μm). The mobile phase was 0.1% formic acid solution and 0.1% formic acetonitrile solution. The flow rate was 0.3 mL·min-1, and the column temperature was set at 30 ℃. HRMS was performed using an electrospray ion source (ESI), and scanned in a positive ion mode by means of full scan/data dependent secondary scan (Full MS/dd-MS2). Compound Discoverer 3.0 software that could be linked to mzCloud network database and local high-resolution mass spectrometry database of traditional Chinese medicine components was used to analyze the data, based on accurate molecular mass, retention behaviors and characteristic ion fragmentation of the compounds, as well as literature information and relevant reference materials. Result::Totally 54 chemical constituents in Citri Sarcodactylis Fructus were identified, including 16 flavonoids, 17 coumarins, 3 limonoids, 6 nucleosides and nucleobases, 2 organic acids, 3 aromatic aldehydes, 1 amino acid and 6 other components. Conclusion::The established UPLC-Q-Orbitrap HRMS method can be used to effectively and rapidly identify main chemical constituents of Citri Sarcodactylis Fructus. The findings provide a methodological reference and theoretical foundation for defining the pharmacodynamic material base and optimizing quality control index of Citri Sarcodactylis Fructus, which is of guiding significance for further development and utilization of the resources.
