Effect of Jianpi Xiao' ai Prescription on Akt/mTOR Signaling Pathways in Colorectal Cells
10.13422/j.cnki.syfjx.20200422
- VernacularTitle:健脾消癌方对结肠癌细胞中Akt/mTOR信号通路的影响
- Author:
Xiao-lan JIAN
1
;
Pu-hua ZENG
1
;
Yong-min LI
1
;
Xiao-ning TAN
1
;
Feng-jiao HE
1
;
Yi-lan JIANG
1
Author Information
1. Innovative Platform of Anti-tumor Chinese Drugs, Hunan Key Laboratory of Chinese Medicine Oncology, The Affiliated Hospital of Hunan Academy of Chinese Medicine, Changsha 410006, China
- Publication Type:Research Article
- Keywords:
Jianpi Xiaoai prescription;
colorectal cancer;
migration and invasion;
protein kinase B (Akt)/mammalian target of rapamycin (mTOR);
phosphorylated
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(7):73-78
- CountryChina
- Language:Chinese
-
Abstract:
Objective::To investigate the effect of drug-containing serum of Jianpi Xiaoai prescription on protein kinase B (Akt)/mammalian target of rapamycin (mTOR) signaling pathways in colorectal cells HCT116. Method::The HTC116 cells were treated by 15%concentration of drug-contained serum, and then the cell migration and invasion were detected by Transwell assay, the protein expression levels of Akt, phosphorylated protein kinase B (p-Akt), mTOR, phosphorylated mammalian target of rapamycin (p-mTOR), ribosomal protein S6 kinase, polypeptide1(S6K1), phosphorylated ribosomal protein S6 kinase, polypeptide1 (p-S6K1), 4E-binding protein1(4EBP1), and phosphorylated 4E-binding protein1(p-4EBP1) in HCT116 cells were detected by Western blot. The control group was treated by untreated serum (15%), and 10%fetal bovine serum(FBS). Result::As compared with the control group, the number of migration and invasion cells was significantly reduced in drug-contained serum group (P<0.01), the expression of Akt had no obvious decrease, p-Akt protein expression was significantly lowered in the drug-contained serum group (P<0.01), the expression of mTOR had no obvious decrease, but p-mTOR protein expression was significantly lowered in drug-contained serum group (P<0.01), the expression of S6K1 had no obvious decrease, but p-S6K1 protein expression was significantly lowered in the drug-contained serum group (P<0.01), the protein expression of 4EBP1 had no obvious decrease, but p-4EBP1 protein expression was significantly lowered in the drug-contained serum group (P<0.01). Conclusion::The anti-tumor mechanism and transfer of Jianpi Xiaoai prescription may be related to inhibiting the activation of Akt/mTOR signaling pathways in colorectal cancer.
