Interferon-γ inducible protein-10 and interleukin 28B gene polymorphism as predictive markers for genotype 4 hepatitis C virus treatment response
https://doi.org/10.47665/tb.37.4.1083
- Author:
Abd El-maksoud, E.A.
1
;
Salem, A.M.
1
;
Maher, A.M.
2
;
Hegazy, M.G.A.
1
Author Information
1. Biochemistry Department, Faculty of Science, Ain Shams University, Abbassia, 11566, Cairo, Egypt
2. Ain Shams Research Institute (MASRI) Cancer Biobank, Faculty of Medicine, Ain Shams University, Cairo, Egypt
- Publication Type:Journal Article
- From:Tropical Biomedicine
2020;37(No.4):1083-1092
- CountryMalaysia
- Language:English
-
Abstract:
HCV genotype 4 dominates the HCV epidemic in Egypt. Drug resistance was the
most serious side effect that reflects bad clinical outcome. Several studies had demonstrated
that baseline serum interferon-γ-inducible-protein 10 (IP-10) levels and interleukin 28B
polymorphisms were associated with the resistance to the standard of care pegylated
interferon alpha and ribavirin (PEG-IFNα/RBV) therapy and development of post-treatment
relapse. Our purpose was to assess the predictive value of combining IP-10 levels and IL28B
genotypes to PEG-IFNα/RBV therapy response in Egyptian chronic HCV infection patients
with genotype 4. Ninety Egyptian patients chronically infected by HCV genotype-4 treated
with pegylated interferon alpha and ribavirin (PEG-IFNα/RBV) therapy were enrolled. Serum
IP-10 levels were determined by enzyme linked immunosorbent assay pre- and post- treatment.
IL-28B (rs12979860 and rs8099917) polymorphisms were performed by PCR-RFLP in all patients.
Overall, 38 patients (42.2%) achieved sustained virologic response (SVR) and 52 (57.8%)
patients have non-viral response (NVR). Pretreatment serum IP-10 mean levels were
significantly lower in patients who achieved SVR than in NVR (P<0.05). CC genotype in IL28B polymorphism (rs12979860) was the favorable genotype as 65.8% achieved SVR, while
TT genotype in IL-28B polymorphism (rs8099917) was the favorable genotype as 81.5%
achieved SVR. Baseline IP-10 was significantly correlated to genotypes CC in rs12979860
and TT in rs8099917. Combined use of serum baseline IP-10 levels with IL-28B polymorphisms
could improve the prediction of SVR to PEG-IFNα/RBV therapy in Egyptian chronic HCV
infection patients with genotype 4.
- Full text:8.2020my1192.pdf
