Construction of a recombinant vaccinia virus expressing Babesia gibsoni thrombospondin-related anonymous protein and evaluation of its immunogenicity in mice
https://doi.org/10.47665/tb.37.4.1029
- Author:
Nakamura, C.
1
;
Liu, M.M.
1
;
Goo, Y.K.
1
,
2
;
Zhang, G.H.
1
;
Jia, H.L.
1
,
3
;
Kumagai, A.
1
;
Zhang, H.S.
1
,
4
;
Zhou, J.L.
1
,
4
;
Nishikawa, Y.
1
;
Xuan, X.N.
1
Author Information
1. National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan&
2. Department of Parasitology and Tropical Medicine, Kyungpook National University School of Medicine, Daegu, 700-422, Republic of Korea
3. State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China
4. Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China
- Publication Type:Journal Article
- From:Tropical Biomedicine
2020;37(No.4):1029-1037
- CountryMalaysia
- Language:English
-
Abstract:
Previously, we have identified a gene encoding thrombospondin-related anonymous
protein of Babesia gibsoni (BgTRAP), and have shown that the antisera raised against
recombinant BgTRAP expressed in Escherichia coli inhibited the growth of parasites. In the
present study, a recombinant vaccinia virus expressing the BgTRAP (VV/BgTRAP) was
constructed. A specific band with a molecular mass of 80 kDa, which is similar to that of
native BgTRAP on the merozoites of B. gibsoni, was detected in the supernatant of VV/
BgTRAP-infected RK13 cells. Mice inoculated with VV/BgTRAP produced a specific antiBgTRAP response. The antiserum against VV/BgTRAP showed reactivity against the native
BgTRAP on parasites. These results indicated that the recombinant vaccinia virus expressing
BgTRAP might be a vaccine candidate against canine B. gibsoni infection.
- Full text:8.2020my1187.pdf
