Giardia duodenalis pathogenicity on immunosuppressed animal model
https://doi.org/10.47665/tb.37.4.1008
- Author:
Wakid, M.H.
1
,
2
;
Toulah, F.H.
3
;
Mahjoub, H.A.
4
;
Alsulami, M.N.
3
;
Hikal, W.M.
5
,
6
Author Information
1. Medical Laboratory Sciences Department, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia&
2. Special Infectious Agents Unit, King Fahd Medical Research Center, Jeddah, Saudi Arabia
3. Biology Department, College of Science, University of Jeddah, Jeddah, Saudi Arabia
4. Biology Department, College of Sciences and Arts, King Abdulaziz University, Rabigh, Saudi Arabia
5. Biology Department, Faculty of Science, University of Tabuk, Tabuk, Saudi Arabia&
6. National Research Centre, Water Pollution Research Department, Giza, Egypt
- Publication Type:Journal Article
- From:Tropical Biomedicine
2020;37(No.4):1008-1017
- CountryMalaysia
- Language:English
-
Abstract:
Giardiasis is the major water-borne diarrheal disease present worldwide caused by
the common intestinal parasite, Giardia duodenalis. This work aims to investigate the effect
of G. duodenalis infection pathogenicity in immunosuppressed animals through
histopathological examination. A total of 45 BALB/c mice were divided into four groups; G1
(negative control), G2 (healthy animals exposed to Giardia); G3 (immunosuppressed animals
exposed to Giardia), and G4 (non-exposed immunosuppressed animals). Our study revealed
that G3 was the most affected group with an infection rate of 100%. The animals showed
general weakness, soft stool, and high death rate with severe histopathological changes in
the duodenum and mild degenerative changes in hepatic tissues. In G2, the maximal lesions
in both duodenum and liver were on the 11th day. We spotted damage in the villi, edema in the
central core, and submucosa, in addition to increased cellular infiltration with inflammation
in lamina propria. The presence of the parasites within the villi and the lumen was clear. Most
of the hepatocytes revealed hydropic and fatty changes, also dilated congested central veins
and edema were observed. G3 changes were more intense than G2 with massive Giardia
trophozoites between the intestinal villi, lumen, and extensive fatty liver degeneration.
Immune suppression plays a significant role in the severity of injury with the Giardia parasites
in duodenum and liver cells.
- Full text:8.2020my1185.pdf
