Morphologic Changes of Normal Skin Fibroblasts According ti the Culture Time.
- Author:
Jong Min KIM
;
Ho Gyun LEE
- Publication Type:Original Article
- Keywords:
Fibroblast Culture;
Morphology
- MeSH:
Actin Cytoskeleton;
Cytoplasm;
Discrimination (Psychology);
Fibroblasts*;
Humans;
Microscopy;
Skin Diseases;
Skin*;
Tissue Donors;
Trypsin
- From:Korean Journal of Dermatology
1995;33(1):59-66
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The morphalogy of fibroblast in culture is important in the discrimination of normal and abnormal cells as well as in recogniring general physiologic status of the cells. There have been many reports on the morphologic clialges in various skin diseases and in response to various drugs. However, we couldnt find any report on the time-sequential morphologic changes of normal fibroblasts in early subculture using light microscopy. OBJECTIVE: The purpose of this study is to describe the time-sequential morphologic changes of normal fibroblhst in early subculture. METHODS: The fibroblaats from 4 normal donors were cultured. We observed the morphologic changes of fibrolMasts in the third passage of subculture using light microscopy at 0-, 30-, 60-, 90-, 120-minutes, 3-, 5-, 24-, 72-hours and days after trypsinization, and electron microscopic exarriioation was done at 21 day-culture. RESULTS & CONCLUSION: Just after trypsinization, the cell were small and round, which divided and increased in number as time went by. At 120-minute culture, many cells had long and thin cytoplasmic elongations and they took stellae,shape at 5-hour culture. At 24-hour culture, several spindle-shaped cells were observec with cell-cell contacts. At 72-hour culture, many spindlle-shaped cells were arranged in medirection, with the appearance of parallel or whorl patterns and showed prominent cell-cell contacts. On electron microscopic examination, there were prominent RER, residial bodies and microfilaments.
