Quantitative in Vivo Detection of Brain Cell Death after Hypoxia Ischemia Using the Lipid Peak at 1.3 ppm of Proton Magnetic Resonance Spectroscopy in Neonatal Rats.
10.3346/jkms.2013.28.7.1071
- Author:
So Yoon AHN
1
;
Hye Soo YOO
;
Jang Hoon LEE
;
Dong Kyung SUNG
;
Yu Jin JUNG
;
Se In SUNG
;
Keun Ho LIM
;
Yun Sil CHANG
;
Jung Hee LEE
;
Ki Soo KIM
;
Won Soon PARK
Author Information
1. Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. wonspark@skku.edu
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Hypoxia-Ischemia, Brain;
Animals;
Newborn;
Magnetic Resonance Spectroscopy;
Flow Cytometry
- MeSH:
Adenosine Triphosphate/*analysis;
Animals;
Animals, Newborn;
*Apoptosis;
Brain/metabolism/pathology;
Cycloheximide/pharmacology;
Hypoxia-Ischemia, Brain/*metabolism/*pathology;
Lactic Acid/*analysis;
Lipids/*analysis;
Magnetic Resonance Spectroscopy;
Membrane Potential, Mitochondrial;
Rats;
Rats, Sprague-Dawley
- From:Journal of Korean Medical Science
2013;28(7):1071-1076
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study was performed to determine the accuracy of proton magnetic spectroscopy (1H-MRS) lipid peak as a noninvasive tool for quantitative in vivo detection of brain cell death. Seven day-old Sprague Dawley rats were subjected to 8% oxygen following a unilateral carotid artery ligation. For treatment, cycloheximide was given immediately after hypoxic ischemia (HI). Lipid peak was measured using 1H-MRS at 24 hr after HI, and then brains were harvested for fluorocytometric analyses with annexin V/propidium iodide (PI) and fluorescent probe JC-1, and for adenosine-5'-triphosphate (ATP) and lactate. Increased lipid peak at 1.3 ppm measured with 1H-MRS, apoptotic and necrotic cells, and loss of mitochondrial membrane potential (DeltaPsi) at 24 hr after HI were significantly improved with cycloheximide treatment. Significantly reduced brain ATP and increased lactate levels observed at 24 hr after HI showed a tendency to improve without statistical significance with cycloheximide treatment. Lipid peak at 1.3 ppm showed significant positive correlation with both apoptotic and necrotic cells and loss of DeltaPsi, and negative correlation with normal live cells. Lipid peak at 1.3 ppm measured by 1H-MRS might be a sensitive and reliable diagnostic tool for quantitative in vivo detection of brain cell death after HI.
