microRNAs expression profiles in Schistosoma japonicum of different sex 14 and 28 days post-infection
https://doi.org/10.47665/tb.37.4.947
- Author:
Ai, L.
1
,
2
;
Hu, W.
1
,
3
;
Zhang, R.L.
2
;
Huang, D.N.
2
;
Chen, S.H.
1
;
Xu, B.
1
;
Li, H.
1
;
Cai, Y.C.
1
;
Lu, Y.
1
;
Zhou, X.N.
1
;
Chen, M.X.
1
,
2
,
4
;
Chen, J.X.
1
Author Information
1. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, National Center for Tropical Diseases Research, WHO Collaborating Center for Tropical Diseases, Key Laboratory of Parasite and Vector Biology, National Health and Family Planning Commission, Shanghai 200025, PR China&
2. Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, PR China&
3. Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai 200438, PR China&
4. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shenzhen Center for Disease Control and Prevention Joint Laboratory for Imported Tropical Disease Control, Shanghai, 200025, PR China
- Publication Type:Journal Article
- From:Tropical Biomedicine
2020;37(No.4):947-962
- CountryMalaysia
- Language:English
-
Abstract:
Different miRNAs are involved in the life cycles of Schistosoma japonicum. The
aim of this study was to examine the expression profile of miRNAs in individual S. japonicum
of different sex before and after pairing (18 and 24 dpi). The majority of differential expressed
miRNAs were highly abundant at 14 dpi, except for sja-miR-125b and sja-miR-3505, in both
male and female. Moreover, it was estimated that sja-miR-125b and sja-miR-3505 might be
related to laying eggs. sja-miR-2a-5p and sja-miR-3484-5p were expressed at 14 dpi in males
and were significantly clustered in DNA topoisomerase III, Rap guanine nucleotide exchange
factor 1 and L-serine/L-threonine ammonia-lyase. Target genes of sja-miR-2d-5p, sja-miR-31-
5p and sja-miR-125a, which were expressed at 14 dpi in males but particularly females, were
clustered in kelch-like protein 12, fructose-bisphosphate aldolase, class I, and heat shock
protein 90 kDa beta. Predicted target genes of sja-miR-3483-3p (expressed at 28 dpi in
females but not in males) were clustered in 26S proteasome regulatory subunit N1, ATPdependent RNA helicase DDX17. Predicted target genes of sja-miR-219-5p, which were
differentially expressed at 28 dpi in females but particularly males, were clustered in DNA
excision repair protein ERCC-6, protein phosphatase 1D, and ATPase family AAA domaincontaining protein 3A/B. Moreover, at 28 dpi, eight miRNAs were significantly up-regulated in
females compared to males. The predicted target genes of these miRNAs were significantly
clustered in heat shock protein 90 kDa beta, 26S proteasome regulatory subunit N1, and
protein arginine N-methyltransferase 1. To sum up, differentially expressed miRNAs may
have an essential role and provide necessary information on clarifying this trematode’s growth,
development, maturation, and infection ability to mammalian hosts in its complex life cycle,
and may be helpful for developing new drug targets and vaccine candidates for schistosomiasis.
- Full text:8.2020my1180.pdf
