Chemokine CXCL12-Induced Calcium Response of Jurkat T Cells under Fluid Shear Stress
10.16156/j.1004-7220.2020.03.11
- VernacularTitle:流体剪切力下趋化因子CXCL12诱导Jurkat T细胞的钙响应机制
- Author:
Bing HU
1
;
Jianhua WU
1
;
Yinchen LING
1
;
Ying FANG
1
Author Information
1. Institute of Biomechanics, School of Bioscience and Bioengineering, South China University of Technology
- Publication Type:Journal Article
- Keywords:
fluid shear stress (FSS);
Jurkat T cell;
calcium response
- From:
Journal of Medical Biomechanics
2020;35(3):E331-E337
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of mechano-chemical coregulation in chemokine-induced calcium response of Jurkat T cells under fluid shear stress (FSS). Methods By using parallel-plate flow chamber combined with fluorescence microscope, the calcium response of Jurkat T cells on CXCL12 was observed to extract the corresponding characteristic parameters under static or flow state, with or without extracellular Ca2+, respectively. Results Immobilized CXCL12 could induce firm adhesion of the circulating Jurkat T cells, and the arrested cells increased with the increase of CXCL12 concentration. Force could trigger the calcium response of Jurkat T cells and sharply raised the activation ratio from 4% up to 75% when the FSS increased from 0 to 20 mPa. Under 20 mPa FSS, extracellular Ca2+ could stimulate quickly the calcium response by shortening the delay time (about 23 s), and enhance calcium intensity by prolonging the climbing time (about 7 s) and half time (about 20 s). Conclusions The cooperation between FSS and extracellular Ca2+ would accelerate and enhance CXCL12-mediated-calcium response of Jurkat T cells, which indicated a fast mechanosensitive pathway through ‘extracellular calcium influx-intracellular calcium store release’. The research results would contribute to understanding the process of T cells activation and providing the clue for relevant pathological and drug research.
