Effects of Concave and Convex Interface on Pluripotency of Mouse Embryonic Stem Cells
10.16156/j.1004-7220.2020.02.10
- VernacularTitle:凹凸界面对小鼠胚胎干细胞多能性的影响
- Author:
Yuanyuan ZHAI
1
;
Yanling SUN
2
,
3
;
Yunping CHEN
2
,
4
;
Jing DU
2
,
3
,
4
;
Yan GONG
1
,
5
Author Information
1. School of Material Science Engineering, Beijing Institute of Fashion Technology
2. Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University
3. Beijing Advanced Innovation Centre for Biomedical Engineering, Beihang University
4. School of Aerospace, Tsinghua University
5. College of Mechanical and Electronic Engineering, Tarim University
- Publication Type:Journal Article
- Keywords:
mouse embryonic stem cells;
substrate;
concave and convex interface;
pluripotency
- From:
Journal of Medical Biomechanics
2020;35(2):E190-E194
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of concave and convex interface on in vitro culture of mouse embryonic stem cells. Methods Mouse embryonic stem cells were cultured on substrate with concave and convex interface. The biological morphology of cell colony was observed. The pluripotency of embryonic stem cells was detected by immunofluorescence and alkaline phosphatase (ALP) staining. Results Embryonic stem cells on concave substrates and convex substrates had higher stereo degree and circularity than those on flat substrates, but it was more obvious on concave substrates. Besides, the expression level of Oct4-GFP and the staining intensity of ALP in embryonic stem cells which were cultured on concave substrates and convex substrates were significantly higher than those on flat basement, especially on concave substrates. Conclusion sCompared with flat substrates, concave substrates and convex substrates had positive effects on the pluripotency maintenance of embryonic stem cells, which could help to maintain pluripotency, but concave substrates had better effects. Changing the substrate curvature could help to maintain pluripotency of embryonic stem cells cultured in vitro. The research findings are of great significance to the study and clinical application of embryonic stem cells.
