Preparation of exosome-coated Bcl-2 siRNA and its inhibitory effect on the growth of pancreatic cancer cells
10.16438/j.0513-4870.2020-1011
- VernacularTitle:负载Bcl-2 siRNA外泌体的制备及其对胰腺癌细胞生长抑制作用
- Author:
Ling ZUO
1
;
Gan QIAO
1
,
2
;
Ming-yue GUO
1
;
Xiu-kun LIN
1
;
Ming-hua LIU
1
,
2
Author Information
1. School of Pharmacy, Southwest Medical University, Luzhou 646000, China
2. Central Nervous System Drug Key Laboratory of Sichuan Province, Luzhou 646000, China
- Publication Type:Research Article
- Keywords:
pancreatic cancer;
Bcl-2;
exosome;
cell proliferation
- From:
Acta Pharmaceutica Sinica
2020;55(12):2918-2923
- CountryChina
- Language:Chinese
-
Abstract:
High expression of Bcl-2 is associated with the development of pancreatic cancer, and downregulation of Bcl-2 is an effective approach for the treatment of pancreatic malignancy. In the present study exosomes were isolated from the cultured medium of human embryonic kidney cells (HEK293) by ultracentrifugation and exosome-coated Bcl-2 siRNA (exosiBcl-2) was synthesized using electroporation. The results showed that the particle size of exosiBcl-2 was 67.3 ± 9.7 nm and the morphology of exosomes displayed a concave ring structure as determined by transmission electron microscopy (TEM). Western blot analysis indicated that exosomal proteins including CD9, CD81, CD63 and TSG101 were highly expressed. Confocal microscopy revealed that exosiBcl-2 was widely distributed in Miapaca-2 cells, and the transfection efficiency of exosiBcl-2 in Miapaca-2 was 77.2% as determined by flow cytometry. Treatment with exosiBcl-2 at a concentration of 100 nmol·L-1 resulted in an inhibitory effect on the growth of Miapaca-2 cells with an inhibition rate of 63%. ExosiBcl-2 treatment can downregulate Bcl-2 and upregulate Bax protein. This study provides evidence that exosiBcl-2 is able to inhibit the growth of pancreatic cancer cells and the nanoparticles have potential to be developed as a novel anticancer agent.
