Recent progress of non-viral vector polyethylenimine in the application of gene delivery
10.16438/j.0513-4870.2020-0740
- VernacularTitle:非病毒型载体聚乙烯亚胺在基因递送应用中的研究进展
- Author:
Cui-ping JIANG
;
Yuan WANG
;
Hai-yan XIAO
;
Hai-yue ZHAO
;
Qiang LIU
- Publication Type:Research Article
- Keywords:
gene vector;
non-viral vector;
gene delivery system;
polyethylenimine;
targeting therapy
- From:
Acta Pharmaceutica Sinica
2020;55(12):2869-2882
- CountryChina
- Language:Chinese
-
Abstract:
In recent years, non-viral gene vectors have attracted great attention for efficient gene delivery due to the advantages, including low toxicity, low immunogenicity and simple preparation. Polyethylenimine (PEI) is one of the typical non-viral gene carriers that have been widely utilized for gene delivery owing to its superior capabilities in gene compression and buffering capacity. This article discusses the processes of gene delivery and the barriers of PEI-based carrier during the gene delivery, such as low biocompatibility, cytotoxicity, lack of specific targeting and insufficient gene release, etc. Therefore, we summarize the multiple approaches for the modifications of PEI in terms of improved biocompatibility, degradability, specific targeting and buffering capacity. Furthermore, we also review on the recent impressive progresses of smart stimuli-responsive PEI carriers, including endogenous stimuli (pH, reactive oxygen species, glutathione, biomolecular, etc), exogenous stimuli (light, temperature, magnetic field, etc) and dual-responsive strategies, which might provide guidance for the development of more efficient and safer non-viral gene vectors.
