Effects of Shikonin on Autophagy and Apoptosis of Human Colon Cancer HCT 116 Cells
- VernacularTitle:紫草素对人结肠癌细胞HCT116自噬和凋亡的影响
- Author:
Xin SHAO
1
;
Xianhong JIANG
2
;
Rui WANG
3
;
Qianghong PU
1
;
Bin HAN
4
;
Fu LIU
4
Author Information
1. Dept. of Pharmacy，Leshan People’s Hospital，Sichuan Leshan 614000，China
2. Dept. of Gastrointestinal Surgery，Leshan People’s Hospital，Sichuan Leshan 614000，China
3. Dept. of Rheumatology and Immunology，Leshan People’s Hospital，Sichuan Leshan 614000，China
4. Dept. of Pharmacy，the Affiliated Hospital of North Sichuan Medical College，Sichuan N anchong 637000，China
- Publication Type:Journal Article
- Keywords:
Shikonin;
Human colon cancer HCT 116 cells;
Autophagy;
Apoptosis
- From:
China Pharmacy
2021;32(1):51-55
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To study the effects of shikonin on autophagy and apoptosis of human colon cancer HCT 116 cells. METHODS：After treating HCT 116 cells for 48 h with shikonin at 0（blank control ）10，20，40 μg/mL，MTT method was used to detect inhibitory rate of cell proliferation. Flow cytometry was used to detect cell apoptosis rate. RT-qPCR assay and Western blotting were respectively used to detect mRNA and protein expressions of microtubule associated protein light chain 3（LC3）and autophagy-related protein Beclin- 1 and p 62. RESULTS ：Compared with blank control ，after treated with 10，20，40 μ g/mL shikonin for 48 h，proliferation inhibitory rate and apoptosis rate of HCT 116 cells were increased significantly （P＜0.05 or P＜ 0.01）. After treated with 10，20，40 μg/mL shikonin for 48 h，mRNA and protein expressions of LC 3，Beclin-1 and p 62 in HCT116 cells were increased to different extents ；except that mRNA expression of LC 3 was not increased significantly after treated with 10 μg/mL shikonin，the difference were statistically significant in other indexes ，compared with blank control （P＜0.05 or P＜0.01）. CONCLUSIONS ：Shikonin can induce the apoptosis of human colon cancer HCT 116 cells and activate its autophagy pathway.
