Effect of GM-CSF on the Embryonic Development and the Expression of Implantation Related Genes of Mouse Embryos.
- Author:
Dong Hoon KIM
;
Duck Sung KO
;
Hoi Chang LEE
;
Ho Joon LEE
;
Hee Gyoo KANG
;
Tai Jeon KIM
;
Won Il PARK
;
Seung Samuel KIM
- Publication Type:Original Article
- MeSH:
Animals;
Blastocyst;
Cell Count;
Embryo Implantation;
Embryonic Development*;
Embryonic Structures*;
Female;
Granulocyte-Macrophage Colony-Stimulating Factor*;
Mice*;
Mice, Inbred ICR;
Oviducts;
Pregnancy;
RNA, Messenger
- From:Korean Journal of Fertility and Sterility
2002;29(2):83-90
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: The purpose of the current series of experiments were to assess the effect of GM-CSF, as a medium supplement, on the development of mouse embryos and the expression of LIF and IL-1beta mRNA. MATERIALS AND METHODS: Mouse 2-cell embryos were collected from the oviducts of 6 weeks old ICR mice at 48 hours after hCG injection. Embryos were cultured in P-1 medium supplemented with mouse GM-CSF(0,1,5,10 ng/ml). The embryo development to blastocysts and hatching blastocysts was assessed and the cell number in blastocyst was also examined. Using RT-PCR, the expressions of LIF and IL-1beta mRNA in blastocyst were evaluated in the GM-CSF supplemented group and control group. RESULTS: In mouse, the addition of GM-CSF increased the percentage of blastocysts(65.5%, 68.6%, 73.0% and 76.1% for control and 1, 5 and 10 ng/ml, respectively), and increased the proportion of hatching blastocysts(35.2%, 36.4%, 43.2% and 53.0% for control and 1, 5 and 10 ng/ml, respectively). The mean cell numbers in blastocyst were significantly increased in GM-CSF supplemented groups compared to control group. LIF and IL-1beta expression in blastocysts were significantly higher in GM-CSF supplemented group than in control group. CONCLUSION: The results of experiment by mouse embryos showed beneficial effects of GM-CSF as a medium supplement. Furthermore, the addition of GM-CSF significantly increased the expression of LIF and IL-1beta in mouse embryos. These results suggest that GM-CSF might be a important molecule in embryo implantation.
