Inhibitory Effect of Swertiamarin on MGC803 Cells in vitro and in vivo
10.3969/j.issn.1008-7125.2019.02.005
- Author:
Weiqiang YANG
1
Author Information
1. Department of Surgery, Central Hospital of Shanghai Jiading District
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Cell Proliferation;
Stomach Neoplasms;
Swertiamarin
- From:
Chinese Journal of Gastroenterology
2019;24(2):86-89
- CountryChina
- Language:Chinese
-
Abstract:
Background: Gastric cancer is one of the most commonly seen malignant tumors. Inhibiting the proliferation of gastric cancer cells is the key for the development of anticancer drugs. Aims: To investigate the effect of swertiamarin on proliferation of MGC803 cells in vitro and in vivo. Methods: MGC803 cells were cultured with different concentrations of swertiamarin (0, 30, 60 and 90 μg/mL) for 48 hours. The cell activity were detected by MTT. The apoptosis rate was measured by Annexin V-FITC/PI assay. The contents of Bax and Bcl-2 were detected by ELISA. MGC803 cell xenograft tumor in BALB/c nude mice was established, and different concentrations of swertiamarin (0, 40, 60 and 80 mg/kg) were injected through caudal vein. Nude mice were killed when the volume of tumor was 1 000 mm3. The initial and final body weights of nude mice were recorded. The protein expression of Ki-67 was detected by immunohistochemistry. Results: Compared with control group, different concentrations of swertiamarin effectively inhibited the proliferation of MGC803 cells, induced cell apoptosis, increased the content of Bax, while the content of Bcl-2 was significantly decreased (P<0.05). In BALB/c nude mice with MGC803 cell xenograft tumor, the volume of tumor, protein expression of Ki-67 decreased significantly in groups injected with different concentrations of swertiamarin than in control group (P<0.05). Conclusions: Swertiamarin is a safe and effective anti-gastric tumor plant agent.
