Expression and Significance of DNA Methyltransferases in Gastric Cancer
10.3969/j.issn.1008-7125.2019.02.004
- Author:
Zhenkai WANG
1
Author Information
1. Endoscopy Center, The Affiliated Nanjing Hospital of TCM of Nanjing Traditional Chinese Medical University
- Publication Type:Journal Article
- Keywords:
DNA Methylation;
DNA Modification Methylases;
Stomach Neoplasms
- From:
Chinese Journal of Gastroenterology
2019;24(2):81-85
- CountryChina
- Language:Chinese
-
Abstract:
Background: DNA methylation plays an important role in the development of gastric cancer, but it needs the modification with DNA methyltransferases (DNMTs). Aims: To investigate the expression and clinical significance of DNMTs in the occurrence and development of gastric cancer. Methods: A total of 80 cases of gastric cancer tissues and corresponding adjacent normal tissues were collected. Immunohistochemistry was used to detect the expressions of DNMT1, DNMT3a, DNMT3b, and their correlations with clinicopathological features of gastric cancer were analyzed. mRNA and protein expressions of DNMT1, DNMT3a, DNMT3b in 4 gastric cancer cell lines and human gastric epithelial cell line were determined by qRT-PCR and Western blotting, respectively. Results: Compared with adjacent normal tissue, the positive expression rate of DNMT1 was significantly increased in gastric cancer (68.8% vs. 10.0%, P<0.01). The positive expression of DNMT1 in gastric cancer tissue was significantly higher than that of DNMT3a and DNMT3b (68.8% vs. 38.8%, 40.0%, P<0.05), while the positive expression of DNMT1 in adjacent tissue was significantly lower than that of DNMT3a and DNMT3b (10.0% vs. 60.0%, 52.5%, P<0.05). The positive expression of DNMT1 was correlated with depth of invasion, lymph node metastasis and TNM stage in patients with gastric cancer (P<0.05), while the positive expression of DNMT3a, DNMT3b were not correlated with clinicopathological features of gastric cancer. The expression of DNMT1 in gastric cancer cells was significantly higher than that in normal gastric epithelial cells, while the expressions of DNMT3a and DNMT3b were significantly decreased. Moreover, the expression of DNMT1 was related to the degree of differentiation of gastric cancer cells (P<0.05). Conclusions: DNMT1 maybe play an important role in the occurrence and development of gastric cancer.
