Effect and Mechanism of Streptococcus gallolyticus Metabolites on Regulating Growth of Colon Cancer Cells
10.3969/j.issn.1008-7125.2019.03.005
- Author:
Jianfeng REN
1
Author Information
1. Department of Gastroenterology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine
- Publication Type:Journal Article
- Keywords:
Cell Migration;
Cell Proliferation;
Colonic Neoplasms;
Streptococcus gallolyticus;
Wnt/β-Catenin Signaling Pathway
- From:
Chinese Journal of Gastroenterology
2019;24(3):148-152
- CountryChina
- Language:Chinese
-
Abstract:
Background: Streptococcus gallolyticus (SG) may alter the colon microenvironment through its metabolites, and transforming the human body into a high-risk state prone to tumor formation. Aims: To investigate the effect and mechanism of Streptococcus gallolyticus metabolites (SGM) on growth of colon cancer cells. Methods: Human colon cancer cell line SW620 was conventionally cultured, and were divided into blank group (group A), healthy intestinal flora group (group B), Bifidobacterium metabolites group (group C), SGM group (group D) and Bifidobacterium metabolites + SGM group (group E). CCK-8 test was used to detect the proliferation of tumor cell. Cell migration ability was detected by scratch test. The protein expression of β-catenin was determined by Western blotting. The mRNA expression of c-myc was measured by real time PCR. Results: Compared with group A, group B, group C, cell proliferation was significantly increased (P<0.05), cell migration ability was significantly increased (P<0.05), protein expression of β-catenin was significantly increased (P<0.05), and mRNA expression of c-myc was significantly increased in group D and group E (P<0.05). No significant differences in above-mentioned indices were found between group D and group E (P>0.05). Conclusions: SG can enhance the proliferation and migration ability of colon cancer cells through its metabolites, which may be through the activating of Wnt/β-catenin signaling pathway. Moreover, Bifidobacterium metabolites cannot inhibit the enhancement of proliferation and migration ability of colon cancer cells by the SGM.
