Preliminary Study on Relationship Between Terminal Ileal Histone Deacetylase 3 Expression and Obesity and Intestinal Flora
10.3969/j.issn.1008-7125.2019.05.002
- Author:
Junjie FAN
1
Author Information
1. Department of Gastroenterology, Shanghai General Hospital, Shanghai Jiao Tong University
- Publication Type:Journal Article
- Keywords:
Diet, High-Fat;
Germ-Free Life;
Histone Deacetylase 3;
Intestinal Flora;
Mice, Inbred C57BL;
Obesity
- From:
Chinese Journal of Gastroenterology
2019;24(5):264-268
- CountryChina
- Language:Chinese
-
Abstract:
Background: Histone deacetylase 3 (HDAC3), which is expressed in liver, muscle and adipose tissue, is closely related to fat metabolism. Furthermore, HDAC3 expressed in intestinal epithelial cells is a critical factor that regulates host-commensal flora relationship and maintains intestinal homeostasis. Aims: To investigate the relationship between HDAC3 expression in terminal ileum and obesity and intestinal flora. Methods: Eight SPF C57BL/6 mice and eight germ-free C57BL/6 mice aged six weeks were randomly placed on a standard chow or a high-fat chow, respectively, for 5 weeks. Changes in body weight were recorded weekly, and the terminal ileum was obtained for detection of HDAC3 protein and mRNA expressions by immunohistochemistry and real-time PCR. Results: Increase of body weight of mice in SPF high-fat diet group was much more than that in SPF normal diet group (P<0.05). Meanwhile, HDAC3 protein and mRNA expressions in terminal ileum in SPF high-fat diet group was significantly higher than those in SPF normal diet group (P<0.05). On the other hand, whether fed with high-fat diet or normal diet, no significant change of body weight occurred in germ-free mice during the 5-week feeding (P>0.05). No significant differences were found in HDAC3 protein and mRNA expressions between germ-free high-fat diet group and germ-free normal diet group (P>0.05). Conclusions: Expression of HDAC3 in terminal ileum might be regulated by intestinal flora and participates in the occurrence of obesity.
