Analysis of Intestinal Mucosal Microbiota and Expression of Oxytocin Receptor in Patients With Irritable Bowel Syndrome
10.3969/j.issn.1008-7125.2019.07.005
- Author:
Junjie CHEN
1
Author Information
1. Department of Gastroenterology, Peace Hospital Affiliated to Changzhi Medical College
- Publication Type:Journal Article
- Keywords:
Intestinal Microbiota;
Irritable Bowel Syndrome;
Receptors, Oxytocin
- From:
Chinese Journal of Gastroenterology
2019;24(7):406-410
- CountryChina
- Language:Chinese
-
Abstract:
Background: Alterations in intestinal microbiota and oxytocin receptors (OTR) expression play important roles in the pathogenesis of irritable bowel syndrome (IBS), however, the underlying mechanisms have not yet been fully elucidated. Aims: To investigate the characteristics of intestinal mucosal microbiota and expression of OTR in patients with IBS and the correlation between intestinal mucosal microbiota and expression of OTR. Methods: Colonic mucosa specimens of IBS-D, IBS-U patients diagnosed by Rome criteria from July 2017 to December 2017 at Peace Hospital Affiliated to Changzhi Medical College were collected, and 13 healthy subjects were served as controls. The species diversity and abundance of intestinal mucosal microbiota were detected by high-throughput sequencing platform. The expression of OTR in colonic mucosa was determined by immunohistochemistry. Results: The mucosal intestinal microbiota of IBS-D group, IBS-U group and control group were mainly consisted of Proteobacteria, Firmicutes, and Bacteroides. No significant differences in chao1 index, shannon index were found among the three groups (P>0.05). Compared with the control group, the abundance of Granulicatella was significantly decreased (H=6.212, P<0.05), the abundance of Tissierella was significantly increased (H=6.688, P<0.05) in IBS group. The abundances of Syntrophaceae, Porphyromonas, Marmoricola, Cardiobacteriales, Cardiobacterium and Cardiobacteriaceae in IBS-D group were significantly higher than those in IBS-U group and control group (P all<0.05). Compared with the control group, the expression of OTR in IBS-D group and IBS-U group were significantly increased (P<0.01). There was a significant positive correlation between Tissierella abundance and colonic mucosal OTR expression (r=0.542, P<0.01). Tissierella, Granulicatella abundance, and colonic mucosal OTR expression were not associated with IBS severity (P>0.05). Conclusions: There is no significant change in intestinal mucosal microbiota diversity in patients with IBS. The abundance of some microbiota is changed, and the abundance of intestinal mucosal microbiota in different subtypes of IBS are different. The expression of OTR in the colonic mucosa of IBS patients is up-regulated and associated with the abundance of Tissierella.
