Quantitative Analysis of Major Shifts in Gut Microbiota Before and After Helicobacter pylori Eradication
10.3969/j.issn.1008-7125.2020.01.004
- Author:
Yuanlin TANG
1
Author Information
1. Department of Gastroenterology, Third People's Hospital of Shenzhen
- Publication Type:Journal Article
- Keywords:
Eradication Therapy;
Gut Microbiota;
Helicobacter pylori;
Real-Time Polymerase Chain Reaction
- From:
Chinese Journal of Gastroenterology
2020;25(1):18-22
- CountryChina
- Language:Chinese
-
Abstract:
Background: Helicobacter pylori (Hp) infection occurs in about half of the world's population, and Hp eradication therapy is an important method for treating Hp infection-related diseases. Aims: To explore the major shifts in gut microbiota before and after Hp eradication by using bismuth-containing quadruple therapy. Methods: Fifty Hp-positive patients in whom the Hp was successfully eradicated by bismuth-containing quadruple therapy from January 2016 to January 2017 at the Third People's Hospital of Shenzhen were enrolled. Using genus-specific primers and genomic DNA extracted from the fecal samples collected before and within 3 days after eradication therapy, a quantitative analysis of the major shifts in gut microbiota was accomplished by real-time PCR. Results: Compared with those before Hp eradication, fecal Enterobacterium and Enterococcus were significantly increased and Bifidobacterium, Bacteroides, Clostridium and total bacteria were significantly decreased after Hp eradication (P<0.05). No significant change was observed in Lactobacillus (P>0.05). Conclusions: Eradication of Hp infection by bismuth-containing quadruple therapy may cause changes in gut microbiota composition and resulting in dysbiosis.
