Detection of serum exosomal miR-148a combined with miR-106a in screening for gastric cancer
10.3969/j.issn.1000-8179.2020.13.183
- Author:
Chao XU
1
Author Information
1. Department of Gastrointestinal Surgery, Fujian Provincal Hospital
- Publication Type:Journal Article
- Keywords:
Exosomes;
Gastric cancer;
MicroRNA
- From:
Chinese Journal of Clinical Oncology
2020;47(13):655-660
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To search for suitable exosomal microRNAs (miRNAs) as tumor biomarkers to assist in the clinical screening of gastric cancer. Methods: The differential expression of miR-148a and miR-106a in cancer and para-carcinoma tissues were determined to assess their potential as biomarkers. These two serum exosomal miRNAs were detected to compare their differential expression in gastric cancer patients and a control group. A total of 37 pairs of postoperative specimens (gastric cancer tissue and para-carcinoma tissue) from gastric cancer patients diagnosed and treated in Fujian Provincial Hospital from September 2017 to September 2018 were selected for histological miRNA detection. Eighty-three patients with newly diagnosed gastric cancer were selected as the gastric cancer group and 78 patients with benign lesions were selected as the control group. Results: Compared with the adjacent tissues, the expression level of miR-148a decreased, the expression level of miR-106a increased, and the value of 2-ΔΔCP (ΔCP=CP(miR-148a)-CP(miR-106a)) decreased in cancer tissues. Compared with the control group, the expression level of serum exosomal miR-106a decreased and the value of 2-ΔΔCP (ΔCP=CP(miR-148a)-CP(miR-106a)) increased in patients with gastric cancer. The area under the curve of 0.844 (95% CI: 0.782-0.905, P<0.001) of 2-ΔΔCP by the combined detection of serum exosomal miRNAs was greater than that of serum exosomal miR-148a or serum exosomal miR- 106a detected separately in distinguishing the gastric cancer and control groups. The cutoff value of 2- Δ Δ CP(Δ CP= CP(miR-148a)-CP(miR-106a)) was 0.3153, the sensitivity was 91%, and the specificity was 64.1%. Conclusions: The 2-ΔΔCP value of the detection of serum exosomal miR-148a combined with miR-106a could be useful in screening for gastric cancer.
