MicroRNA-124 mediates hepatocellular carcinoma malignancy by targeting caveolin-1
10.3969/j.issn.1000-8179.2020.05.188
- VernacularTitle: MicroRNA-124 靶向调控 caveolin-1 介导肝癌细胞恶性行为的研究*
- Author:
Daqing KOU
1
Author Information
1. Department of Clinical Laboratory, First Affiliated Hospital of Dalian Medical University
- Publication Type:Journal Article
- Keywords:
Caveolin-1;
Hepatocellular carcinoma;
MiR-124
- From:
Chinese Journal of Clinical Oncology
2020;47(5):227-230
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effect of microRNA-124 (miR-124)/caveolin-1 in the progression of hepatocellular carcinoma (HCC). Methods: The expression levels of miR-124 and caveolin-1 were evaluated in HCC tissues, adjacent tissues, and cell lines by qRT-PCR. A dual-luciferase reporter gene assay was used to confirm the relationship between microRNA-124 and caveolin-1. MicroRNA-124 mediated caveolin-1 levels, which was determined by qRT-PCR and Western blot analyses. CCK-8, colony formation, and transwell assays were used to evaluate the proliferation and invasion of HCC cells. Results: A low level of miR-124 together with a high level of caveolin-1 was found in HCC tissues and MHCC97H cells compared with control cells. Caveolin-1 was determined to be the target gene of miR-124, and alterations of miR-124 could modulate caveolin-1. Cells transfected with a miR-124 mimic in MHCC97H cells inhibited proliferation and invasion, whereas upregulation of caveolin-1 promoted proliferation and invasion. MHCC97L cells transfected with a miR-124 inhibitor showed increased proliferation and invasion, whereas cells transfected with siCaveolin-1 showed decreased proliferation and invasion. Patients with HCC with higher miR-124 and lower caveolin-1 levels lived longer than patients with lower miR-124 and higher caveolin-1 levels. Conclusions: MiR-124 mediated the proliferation and invasion of HCC by targeting caveolin-1.
