Optimization of 18F-FHBG automated synthesis method and quality assessment.
10.13929/j.1003-3289.201806130
- Author:
Liu YANG
1
Author Information
1. Department of Nuclear Medicine, the First Affiliated Hospital of Zhengzhou University
- Publication Type:Journal Article
- Keywords:
Fluorine radioisotopes;
Isotope labeling;
Tomography, emission computed
- From:
Chinese Journal of Medical Imaging Technology
2019;35(3):417-422
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To optimize the synthesis method of 18F-FHBG, and to investigate the quality stability of the synthesized 18F-FHBG. Methods The automated synthesis process of 18F-FHBG was self-designed, and the N2-(p-methoxyphenyldiphenylmethyl)-9-([4-toluenesulfonyl]-3-p-methoxyphenyl diphenylmethoxy) was directly labeled with 18F- by the nucleophilic reaction, then 18F-FHBG product was obtained by elution and purification. The synthesized time was recorded. The synthesis efficiency, radiochemical purity and the stability of the products were evaluated. The biodistribution of 18F-FHBG and the specificity of imaging characteristics were also observed, respectively. Results The synthesis time of 18F-FHBG was 19-25 min, the uncorrected synthesis efficiency was (19.00±5.00)% (n>10), and the radiochemical purity was all >97% after 5, 30, 60, 90, 120 min and 6 h at room temperature. The results of biodistribution experiments showed that 18F-FHBG was mainly distributed in normal Kunming mice in the liver, gastrointestinal tract and kidney, and the brain tissue was less ingested. PET/CT imaging showed that after injection of 18F-FHBG into New Zealand white rabbits, the radioactivity distribution was faster throughout the body, and the imaging effect was good at 60 min. Conclusion The optimized automatic synthesis method can quickly and efficiently synthesize 18F-FHBG with high purity and stability, which is suitable for PET/CT whole body imaging.
