Immunomodulators Extracted from Korean - style Fermented Soybean Paste and Their Function . 1 . Isolation of B Cell Mitogen from Korean - style Fermented Soybean Paste.
- Author:
Bong Ki LEE
;
Yun Soo JANG
;
Sook Yi YI
;
Kun Sub CHUNG
;
Shin Yang CHOI
- Publication Type:In Vitro ; Original Article
- Keywords:
IL-6;
IL-12;
IFN-r;
TNF-a;
lectin;
B cell mitogen
- MeSH:
Agglutination;
Ammonium Sulfate;
Animals;
B-Lymphocytes;
Cell Proliferation;
Fermentation;
Filtration;
Glycoproteins;
Immunologic Factors*;
Interleukin-12;
Interleukin-6;
Lectins;
Lymphocytes;
Macrophages;
Membranes;
Mice;
Soybeans*;
T-Lymphocytes;
Ultrafiltration
- From:Korean Journal of Immunology
1997;19(4):559-570
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Responses of mouse lymphocytes to the soybean paste fermented by Korean traditional fashion was examined to clarify its effects in cytokine production in vitro. A fraction of the soybean paste (KFSP-100) was prepared by precipitation with ammonium sulfate and by filtration through ultrafiltration membrane. KFSP-100 were added into cultures of fresh mouse splenic cells in vitro. KFSP-100 significantly enhanced the amount of IL-6 and TNF-a produced by macrophages and IL-6 and IFN-r produced by lymphocytes. Production of IL-12 by macrophages was not much affected by KFSP-100 treatments. The most noticeable finding was the fact that lymphocytes treated with KFSP-100 proliferated to an exceeding numbers (more than 10 times to the control) in 72 hours. The KFSP-100-induced proliferative response was specific to B cells since almost all of the KFSP-100-induced cells in the cultures of splenic cells were B cells. Furthermore, such a proliferative responses were equally observed only in cultures of purified B cells but not in cultures of T cells. In thermostability test, the biologically active components of the KFSP-100 is assumed to be either linear protein or glycoprotein. KFSP-100 did not induce agglutination of lymphocytes demonstrated by lectins in the same cells. These observations suggest that KFSP-100 may be a novel mitogen for B lymphocytes. The component (s) responsible for the B cell proliferation in KFSP-100 might be a factor gained by natural fermentation. None of the fractions of not fermented soybean paste prepared by the same methods demonstrate the same effect.
