Effect of Nitric Oxide on the Viability of Bone Marrow - Derived Cultured Mast Cells.
- Author:
Hun Taeg CHUNG
;
Rae Kil PARK
;
Chang Duk JUN
;
Byung Min CHOI
;
Seog Jae LEE
- Publication Type:Original Article
- Keywords:
Mast cell;
Nirtic oxide;
Apoptosis
- MeSH:
Apoptosis;
Bone Marrow*;
Cell Cycle;
Cell Death;
Cytokines;
DNA Fragmentation;
Interleukin-3;
Mast Cells*;
Nitric Oxide*;
Nitroprusside;
S Phase;
Stem Cell Factor
- From:Korean Journal of Immunology
1997;19(4):595-600
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
It is well established that mast cell proliferation and maturation are regulated by two principle cytokines, IL-3 and the c-kit ligand stem cell factor (SCF). Previous reports have demonstrated that bone marrow-derived IL-3-dependent mast cells exhibit the characteristic apoptosis on removal of IL-3. To know how the number of mast cells is controlled, we observed the effects of nitric oxide (NO) on the murine bone marrow-derived cultured mast cells (BMCMC). Apoptosis was measured by the analysis of flow cytometric data and electrophoretic evidence of DNA fragmentation. Our data showed that sodiurn nitroprusside (SNP)-a NO releasing substance- induced apoptosis in BMCMC. Cell cycle analysis showed that the number of the G,/G, and S phase decreased markedly, while the percentage of cell in G,/M phase was increased. Also, SNP alone induced cell death, whereas SNP in combination with SCF markedly decreased cell death of BMCMC. SNP-induced apoptosis was partially inhibited by the treatment of BMCMC with SCF. Our results suggest that NO might have sorne role in the regulation of the number of mast cells.
