The Effect of Recombinant Human Growth Hormone on Neutrophil Activity in Children.
- Author:
Hun Taeg CHUNG
;
Jong Duck KIM
;
Hoon RYU
;
Du Young CHOI
- Publication Type:Original Article
- Keywords:
Growth hormone;
Polymorphoneuclear leukocyte activity
- MeSH:
Child*;
Growth Hormone;
Human Growth Hormone*;
Humans*;
Leukocytes;
Neutrophils*;
Plasma;
Radioimmunoassay;
Respiratory Burst;
Superoxides
- From:Korean Journal of Immunology
1997;19(4):629-638
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
We investigated the acute effect of recombinant human growth hormone (rhGH) on the activity of polymorphoneuclear leukocyte (PMN). We selected 6 patients of growth hormone deficient and 5 normal control children. In both groups, 0.15 IU/kg of rhGH was administered subcutaneously. The plasma growth hormone level were measured by radioimmunoassay on 0, 2, and 6 hours after administration of rhGH. To determined PMN activity, peripheral blood PMN were separated by discontinuous density-gradient centrifigation. Isolated PMN were stimulated hy fMLP and PMA and then respiratory burst activity of PMN was determined. The average growth hormone level of growth horrnone deficient and normal group were increased to the level of 41.6+/-23.7 and 96.3+/-46.5 ng/ml respectively, 2 hours after rhGH injection and decreased to the level of 18.5+/-10.6 and 42.2+/-5.5 ng/ml respectively, 6 hours after rhGH injection. Superoxide (O ) production by PMN which was stimulated by PMA was increased from 9.98+/-5.18 to 38.67+/-19.19 (x 10'cpm) after 6 hours of rhGH injection in control group children. It seemed that administration of the rhGH do not made a any effects acutely on PMN activity in growth hormone deficient group. But in a normal control children, extemal administration of rhGH acutely increased activity of PMN.
