Application of multimodal imaging in vivo monitoring transplanted mesenchymal stem cells
10.13929/j.1003-3289.201903130
- Author:
Yue HU
1
Author Information
1. Department of Interventional, The Third Affiliated Hospital, Institute of Interventional Radiology, Sun Yat-sen University
- Publication Type:Journal Article
- Keywords:
Bioluminescence;
Liver;
Magnetic resonance imaging;
Mesenchymal stem cells;
Rats
- From:
Chinese Journal of Medical Imaging Technology
2019;35(11):1622-1626
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the ability of combining bioluminescence imaging (BLI) and MRI to make real-time and non-invasive tracer of mesenchymal stem cells (MSCs) transplanted into damaged liver of rats; and to study the functional repair effect of transplanted MSCs on injured liver in rats. Methods: Rat model with acute liver injury were transplanted the MSCs of labeled luciferase and SPION. Rat plasma aminotransferase levels and liver pathology change were analyzed, and the repair effect of MSCs on liver function injury were observed. The time course of MSCs accumulation in rat liver was observed by BLI and MRI. In situ immunohistochemical staining of rat liver tissue sections was performed. Results: Plasma aspartate aminotransferase and alanine aminotransferase levels were significantly lower on days 5, 9 and 10 after transplantation than in the control group (all P<0.05). On the first day after the transplantation of MSCs, the bioluminescence intensity in the liver area of rats was significantly enhanced, and the T2*WI standardized signal intensity was significantly reduced, and then gradually returned to the pre-transplantation level 10 days after the transplantation. The number of luciferase-positive MSCs decreased with the decrease of bioluminescence intensity. Conclusion: Transplantation of MSCs has a good repair effect on the damaged liver function of rats. In addition, BLI combined with MRI is used for real-time and non-invasive tracing of MSCs transplanted into the injured liver of rats.
