Automatic quantitative assessment of viable myocardium after ischemia-reperfusion using myocardial contrast echocardiography based on neutrosophic similarity score system
10.13929/j.issn.1003-3289.2020.04.007
- VernacularTitle: 基于中智相似积分算法心肌超声造影系统自动定量分析缺血再灌注大鼠存活心肌
- Author:
Zhichao SUN
1
Author Information
1. Department of Ultrasound, the Second Affiliated Hospital of Harbin Medical University
- Publication Type:Journal Article
- Keywords:
Computer-aided;
Echocardiography;
Myocardial reperfusion;
Neutrosophic similarity score
- From:
Chinese Journal of Medical Imaging Technology
2020;36(4):508-513
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the accuracy of automatic quantitative assessment for viable myocardium of rat ischemic-reperfusion (I/R) models using myocardial contrast echocardiography (MCE) based on neutrosophic similarity score (MCE-NSS) system. Methods: Totally 12 SD rats underwent 30 min (I/R-30 group, n=6) or 45 min (I/R-45 group, n=6)occlusion of the left anterior descending coronary artery followed by reperfusion. MCE was performed before and immediately, 7, 14 and 28 days after operation. The left ventricular myocardium was divided into 18 segments, and the standardized contrast intensity (CI) and wall thickness (WT) of each myocardial segment were automatically calculated using MCE-NSS system after identifying the endocardial and epicardial boundary. The segments with WT<0.3 were considered as the dangerous segments during occlusion, and then the dangerous central region (WT<0.3 and CI<-54 Pix), peripheral region (WT<0.3 and CI>-54 Pix) and recovered region (WT>0.3 and CI>-54 Pix) were defined by MCE-NSS system after reperfusion. The changes of the central areas, peripheral areas and recovery areas were observed immediately, 7, 14 and 28 days after operation. The heart of rats were harvested 28 days after I/R, and myocardial sections were stained with Masson staining and immunohistochemistry to calculation of infarct area and microvessel density (MVD), and the correlation between which and the infarct area measured by MCE-NSS system were analyzed. Results: ①For area changes of dangerous sections, no significant difference of the dangerous central region was found between 2 groups immediately and 7, 14 and 28 days after operation (all P>0.05), while the dangerous peripheral region gradually decreased and the recovered region gradually increased 7, 14 and 28 days after reperfusion(all P<0.01). The areas of the dangerous central region in I/R-45 group were all significantly larger than in I/R-30 group (all P<0.01), but there was no significant difference of the dangerous peripheral and recovered region between 2 groups (all P>0.05). ②The areas of the dangerous central regions were positively correlated with infarct size calculated by Masson staining (r=0.81, P<0.01), and the CI value of the dangerous peripheral regions was positively correlated with MVD obtained by immunohistochemistry (r=0.86, P<0.01). Conclusion: MCE-NSS system can be used to automatically and quantitatively assess the regional systolic function and microcirculation in left ventricle, and then identify viable myocardium of rats after I/R.
