Determination of aflatoxins in fruit traditional Chinese medicines by rapid-resolution liquid chromatography tandem mass spectrometry
- Author:
Jian-Ming LI
1
Author Information
1. Guangzhou Institute for Drug Control
- Publication Type:Journal Article
- Keywords:
Aflatoxins;
Fruit;
RRLC-MS/MS;
Traditional Chinese medicine
- From:
Chinese Pharmaceutical Journal
2012;47(1):65-68
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To develope a method for determination of four aflatoxins (B1, B2, G1, G2) in fruit traditional Chinese medicines by rapid-resolution liquid chromatography coupled with electrospray ionization triple quadruple tandem mass spectrometry. METHODS: After being extracted with 70% methanol and purified by immunoaffinity columns, the four aflatoxins were separated on a Shim-pack XR-ODS column using gradient elution with the mobile phase of CH3CN-CH3OH-H2O mixtures monitored under MRM mode in ESI positive scan. RESULTS: There was a good linear relationship over the range of 0.102 1-10.21 ng · mL-1 for AFB1, 0.061 2-7.65 ng · mL-1 for AFB2, 0.193-9.65 ng · mL-1 for AFG1, 0.121-7.55 ng · mL-1 for AFG2 (r > 0.999 8), respectively. The average recoveries ranged from 77.0%-102.4%. CONCLUSION: This method provides a simple and feasible way to determine aflatoxins.
