Identification of metabolites of senecionine, a hepatotoxic pyrrolizidine alkaloid, by liquid chromatography/tandem mass spectrometry
- Author:
Ai-Zhen XIONG
1
Author Information
1. The MOE Key Laboratory for Standardization of Chinese Medicines
- Publication Type:Journal Article
- Keywords:
Hepatotoxic pyrrolizidine alkaloid;
In vivo metabolism;
Senecionine;
Tandem mass
- From:
Chinese Pharmaceutical Journal
2012;47(1):54-60
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To investigate the metabolic profile of senecionine in rats. METHODS: Liquid chromatography-tandem mass spectrometry (LC-MSn) mass spectrometry was used for the analysis of senecionine and its metabolites in rats bile, urine, and feces after a single oral dose of senecionine. RESULTS: In total 38 metabolites were identified, including oxidation products, hydroxylation products, hydrolysis products, sulfation products, glucuronidation products, and GSH-conjugations. And metabolites were found to be more abundant in bile and urine than those in feces. CONCLUSION: Senecionine undergoes extensive metabolism in rats.
