Determination of 5-hydroxylansoprazole/lansoprazole sulfone by HPLC-MS/MS in incubation system in vitro
- Author:
Cheng-Liang ZHANG
1
Author Information
1. Department of Pharmacy
- Publication Type:Journal Article
- Keywords:
5-hydroxylansoprazole;
Cytochrome P450 enzymes;
HPLC-MS/MS;
Lansoprazoe sulfone;
Lansoprazole
- From:
Chinese Pharmaceutical Journal
2012;47(1):49-53
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a HPLC-MS/MS method for determing 5-hydroxylansoprazole/lansoprazole sulfone, and optimize the incubated conditions for rat liver microsomes. METHODS: 5-Hydroxylansoprazole/lansoprazole were determined by HPLC-MS/MS. Single factor design was used to optimize the incubated conditions and the enzymes kinetics value was evaluated by graphical analysis with Lineweaver-Burk double reciprocal plots. RESULTS: The linear range of 5-hydroxy lansoprazole and lansoprazole sulphone in liver microsome incubation system were 5.57-2 520 and 5.42-2 480 ng · mL-1, respectively. The optimal incubated conditions were 10 μmol · L-1 lansoprazole, 0.16 mg liver microsomes, and 10 min incubation, respectively. CONCLUSION: The HPLC-MS/MS method is accurate and suitable for the determination of 5-hydroxy lansoprazole and lansoprazole sulfone in rat liver microsomes. The incubated condition can be applied for study in the drug interaction with lansoprazole.
