Determination of sufentanil in plasma of patients receiving intravenous combined anesthesia by LC-MS/MS
- Author:
Yu LIANG
1
Author Information
1. Department of Pharmacy
- Publication Type:Journal Article
- Keywords:
Composite anesthesia;
LC-MS/MS;
Plasma concentration;
Sufentanil
- From:
Chinese Pharmaceutical Journal
2012;47(2):146-149
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a LC-MS/MS method for the determination of sufentanil plasma concentration in patients receiving intravenous combined anesthesia. METHODS: After adding 2 mol · L-1 NaOH as the basification reagent, sufentanil was extracted from plasma by ether. Then sufentanil was determined by LC-MS/MS using fentanyl as the internal standard. Separation was carried on a Diamonsil C18 column (4.6 mm × 150 mm, 5 μm) with a mobile phase of methanol-5mmol · L-1 ammonium acetate-acetic acid (74: 26: 0.5) at the flow rate of 0.5 mL · min-1. TIS source was applied and operated in positive ion mode. Quantitative determination was performed using multiple reaction monitoring (MRM) of m/z 387.2 → m/z 238.2 for sufentanil, and m/z 337.4 → m/z 188.2 for fentanyl. RESULTS: The calibration curve was in good linearity over the range of 0.01-1.00 μg · L-1(r=0.9981). The extraction recovery for sufentanil were more than 64.2%, while the intra-day and inter-day precision (RSD) were lower than 11.6%. CONCLUSION: The method is shown to be simple, rapid, accurate and sensitive, which can be applied to the determination of sufentanil and the pharmacokinetics study of sufentanil in patients receiving intravenous combined anesthesia. Copyright 2012 by the Chinese Pharmaceutical Association.
