Uptake of album nanoparticles by liver cells
- Author:
Zhao-Ying JIN
1
Author Information
1. Department of Pharmacy
- Publication Type:Journal Article
- Keywords:
Album nanoparticles;
Endocytosis;
Fluorescence intensity
- From:
Chinese Pharmaceutical Journal
2012;47(2):113-118
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To study the endocytosis of albumin nanoparticles by L02 cells. METHODS: Calcein human serum album nanoparticles (calcein HSA-NPs) were prepared by a desolvation technique with calcein as a fluorescent probe. The particle size distribution, in vitro release, cell uptake, intracellular drug distribution, and cytotoxicity were determined. RESULTS: The prepared calcein HSA-NPs with an average diameters of 203.3 nm showed sustained release profile in vitro. The L02 cell uptake of nanoparticles showed time, concentration, and temperature dependency. HSA-NPs could significantly improve the transmembrane transport capacity of calcein as a water-soluble small molecule (P < 0.01). Clathrin-mediated endocytosis and macropinocytosis were the main routes by which calcein HSA-NPs entered into cells. After entering into cells, nanoparticles were located in the intracellular space outside the nucleus, mainly in the lysosomes. HSA-NPs had no cytotoxicity basically. CONCLUSION: Calcein HSA-NPs can be used as an effective vehicle to study the mechanism of endocytosis. Copyright 2012 by the Chinese Pharmaceutical Association.
