Effects of sodium tanshinone II A sulfonate on Ang II-induced cardiomyocyte oxidative stress
- Author:
Le YANG
1
Author Information
1. Tongji Hospital of Huazhong University of Science and Technology
- Publication Type:Journal Article
- Keywords:
Cardiomyocyte hypertrophy;
NADPH oxidase;
Reactive oxygen species;
Sodium tanshinone II A sulfonate
- From:
Chinese Pharmaceutical Journal
2012;47(4):270-274
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To observe the effects and mechanisms of sodium tanshinone II A sulfonate (STS) on angiotensin II (Ang II)-induced cardiomyocyte oxidative stress. METHODS: In the primary culture of neonatal rat cardiomyocytes, the content of reactive oxygen species (ROS) was measured by 2, 7-dichlorofluorescein diacetate (DCFH-DA). 8-hydroxydeoxyguanosine level in the supernatant was measured by ELISA. As indexes of cardiomyocyte oxidative stress, the cellular contents of MDA and SOD, cell vialibity and LDH release were measured. NADPH oxidase (NOX) activity was measured by chromatometry. The expressions of p47phox was assessed using Western blot. RESULTS: STS can decrease Ang II-induced elevations of ROS level and oxidative stress, and inhibit the expression of p47phox and NOX activity. CONCLUSION: The inhibitory effects of STS on Ang II-induced cardiomyocyte oxidative stress may be associated with depressing NOX signaling pathway via down regulation of p47phox expression. Copyright 2012 by the Chinese Pharmaceutical Association.
