Research on change of S180 tumor cell membrane function by Auricularia polytricha polysaccharides
- Author:
Guang-Lei SONG
1
Author Information
1. Institute of Food and Biological Engineering
- Publication Type:Journal Article
- Keywords:
Antitumor;
Auricularia polytricha;
Mechanism;
Polysaccharide
- From:
Chinese Pharmaceutical Journal
2012;47(4):255-261
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: Auricularia polytricha is paid great attention to as an anticancer food, of which polysaccharide is an important functional component. The anticancer mechanism of Auricularia polytricha polysaccharide is critically important to its development and usage. METHODS: Five polysaccharides were isolated using counter-current chromatography and DEAE column chromatography from the wild Auricularia polytricha. RESULTS: AAPS1, AAPS2 and AAPS3 were light yellow floc, and AAFRB and AAFRC were white powder. The activities of the 5 polysaccharides on S180 sarcoma cell membrane were analyzed, and they could significantly change the characteristic of the tumor cell membrane, resulting in more free fatty acids. Among the 5 polysaccharides, AAPS2 showed the highest activity. The polysaccharides significantly lowered the levels of sialic acid (SA) in the cell membrane of tumor-bearing mice (P < 0.01), and AAPS2 had the highest activity than those of others. The polysaccharide AAPS2 could significantly alter membrane fluidity (P < 0.05). CONCLUSION: The anti-tumor mechanism may be related to the membrane glycoprotein and glycolipids chain terminal residues SA, and the peroxidation of lipid in cell membrane. Copyright 2012 by the Chinese Pharmaceutical Association.
