Study on the metabolites of phenoprolamine hydrochloride in human body and human liver microsomes by LC-MS/MS
- Author:
Lin HUANG
1
Author Information
1. Department of Pharmacy
- Publication Type:Journal Article
- Keywords:
Extrahepatic metabolic pathway;
Human liver microsomes;
LC-MS/MS;
Phenoprolamine hydrochloride
- From:
Chinese Pharmaceutical Journal
2012;47(5):371-375
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To study the metabolites of phenoprolamine hydrochloride in human body and in human liver microsomes. METHODS: Two volunteers were administrated with 60 mg of phenoprolamine hydrochloride, and plasma samples were collected in specified time points; phenoprolamine hydrochloride was incubated in human liver microsomes. All the metabolites were identified with the LC-MS/MS system. RESULT: One key metabolite was detected in human plasma which m/z was 212.1. Six metabolites were detected in human liver microsomes and the m/z were separately 360.1, 342.2, 415.1, 281.4 and 330.2. Based on metabolite analysis, several metabolic pathways in human body and human liver microsomes were proposed. CONCLUSION: The metabolites of phenoprolamine hydrochloride in human body were different with that in human liver microsomes. We assumed phenoprolamine hydrochloride would metabolize mainly through other pathway but not in the liver. Copyright 2012 by the Chinese Pharmaceutical Association.
