Preparation of pH-sensitive procationic liposomes and tumor distribution study in mice
- Author:
Pei-Pei RONG
1
Author Information
1. Department of Pharmacy
- Publication Type:Journal Article
- Keywords:
Hydroxycamptothecin pH-sensitive;
Procationic;
Tumor distribution
- From:
Chinese Pharmaceutical Journal
2012;47(6):444-447
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: The pH-sensitive procationic liposomes were prepared, with hydroxycamptothecin (HCPT) as model drug. METHODS: The cationic liposomes were prepared by film dispersion method. The encapsulation efficiency was determined after removing unentrapped HCPT by Sephadex-G50 chromatographic column. Carboxymethyl chitosan was used to coat the cationic liposomes, then the liposomes were extruded through high pressure homogenizer. Zetasizer was used to determine the zeta potential, average size of the HCPT liposomes. pH sensitivity analysis was made through experiments of red blood cell hemolysis and tumor distribution is analysised. RESULTS: The size of liposomes was around 96 nm and drug encapsulation efficiency was 78.5%. The membrane fusion of red blood cells with procationic liposomes was significantly different between pH 6.5 and pH 7.4 and the in vivo experiments in tumor-bearing mice suggested that they had a high tumor distribution. CONCLUSION: The pH-sensitive procationic nanoliposomes were easy to prepare and were expected to be a promising anti-tumor drug delivery system. Copyright 2012 by the Chinese Pharmaceutical Association.
