Effects of different triptolide formulations given by MEMS micro-needles on skin injury of rheumatoid arthritis rats
- Author:
Gui CHEN
1
Author Information
1. School of Life and Science
- Publication Type:Journal Article
- Keywords:
Lactate dehydrogenase;
Micro-needle;
Rheumatoid arthritis model;
Skin injury;
Triptolide
- From:
Chinese Pharmaceutical Journal
2012;47(6):435-438
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To study the effects of different triptolide formulations given by micro electro mechanical systems (MEMS) micro-needles on skin injury of rheumatoid arthritis rats . METHODS: Three formulations of triptolide were prepared including the aqueous solution of 20% propylene glycol, liposomes, and liposomes hydrogel patch. Rats with rheumatoid arthritis induced by collagen II were used, and 100 and 200 μm MEMS micro-needles treatment was performed on left and right sides of rat skin. The lactate dehydrogenase (LDH) activities were measured by ultraviolet spectro-photometric method. RESULTS: After processed by MEMS micro-needle, the LDH activities of RA rat skin did not change obviously (P > 0.05). All triptolide formulations increased LDH activities (P < 0.05), and the increasement of solution group was largest (P < 0.001), followed by lipsomes group (P < 0.01) and liposomes hydrogel patch (P < 0.05). Nevertheless, there was no significant difference between left and right sides of rat skin (P > 0.05). CONCLUSION: Both 100 and 200 μm MEMS micro-needles were safe for enhancement of transdermal delivery in rheumatoid arthritis rats. The skin injury was observably relieved when triptolide was prepared as liposomes hydrogel patch which is a perfect formulation for transdermal delivery of triptolide. Copyright 2012 by the Chinese Pharmaceutical Association.
