Targeting gene delivery to prostatic cancer with mAb-conjugated polyamidoamine dendrimers
- Author:
Xin WU
1
Author Information
1. Department of Pharmacy
- Publication Type:Journal Article
- Keywords:
Dendrimer;
Gene vector;
Monoclonal antibody;
Polyethylene glycol;
Prostatic cancer;
Targeting
- From:
Chinese Pharmaceutical Journal
2012;47(6):418-422
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: Aim to construct a novel polymer gene delivery system based on a new kind of dendrimer-polyamidoamine (PAMAM). With the modification of polyethylene glycol (PEG) and YPSMA-1 monoclonal antibody (mAb), PAMAM-PEG-mAb was successfully synthesized as a novel gene vector targeting to the prostatic cancer. METHODS: NMR was used to characterize PAMAM-PEG-mAb. The cellular uptake and prostatic cancer (PCa) distribution experiments were employed to explore its biological characteristics and PCa cell (PC3 and LNCaP) targeting ability. RESULTS: NMR results demonstrated the successful synthesis of PAMAM-PEG-mAb. The cellular uptake of vectors was concentration-dependent. The gene expression in vitro indicated that the modification of mAb could increase the gene expression efficiency and PCa targeting ability of PAMAM vectors to LNCaP ( PSMA overexpressing prostate cancer cells). CONCLUSION: PAMAM-PEG-mAb is a potential gene delivery vector targeting to PCa. Copyright 2012 by the Chinese Pharmaceutical Association.
