Extraction optimization of anthraquinone from aerial part of Rheum officinale Baill based on Plackett-Burman design combined with CCD response surface methodology
- Author:
Yu-Zhu TAN
1
Author Information
1. Chengdu University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Central composite design;
Emodin;
Extraction process;
Plackett-Burman;
Rheum officinale Baill;
Total anthraquinone
- From:
Chinese Pharmaceutical Journal
2012;47(6):410-414
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To optimize the process of extacting effective constituents from aerial part of Rheum officinale Baill by Plackett-Burman design combined with CCD response surface methodology. METHODS: In Plackett-Burman combined with CCD response surface design, the independent variables were concentration of ethanol, extraction time, and solvent fold. The dependent variables were contents of total anthraquinone and rheum emodin. RESULTS: The optimal extraction process of total anthraquinone was as follows: 67.25% ethanol, 90 min for reflux per time, 28:1 fold of solvent and 2 times of extraction; the optimal extraction process of emodin was 85% ethanol, 90 min for reflux per time, 40 fold of solvent and 2 times of extration. CONCLUSION: The optimal process is simpleand convenient for extracting aerial part of Rheum officinale Baill with high precision and predictability. Copyright 2012 by the Chinese Pharmaceutical Association.
