Simultaneous determination of six index components in Scrophularia ningpoensis by HPCE
- Author:
Le HAN
1
Author Information
1. Nanjing University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Acteoside;
Cinnamic acid;
HPCE;
Iridoid glycoside;
Scrophularia ningpoensis
- From:
Chinese Pharmaceutical Journal
2012;47(7):555-559
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a high performance capillary electrophoresis (HPCE) method for simultaneous determination of catalpol, aucubin, harpagide, harpagoside, acteoside and cinnamic acid in Scrophularia ningpoensis of different batches. METHODS: 60 mmol · L-1 sodium borate was used as buffer solution (pH 9.3), uncoated fused silica capillary (64.5 cm × 75 μm id, 56 cm of effective length) was used, separation voltage was 20 kV, detection wavelength was 210 nm, column temperature was maintained at 25°C, and the sample was injected at 50 kPa, 6s. RESULTS: Six index components showed good linearity (r > 0.9971) in the range of the tested concentration, and the average recoveries of the method were between 97.05%-103.43%. CONCLUSION: The method is simple, accurate and reproducible, and can be used for quality control of Scrophularia ningpoensis. Copyright 2012 by the Chinese Pharmaceutical Association.
