HPLC analysis of monacolin J in biosynthesis
- Author:
Ru-Sheng BAO
1
Author Information
1. Zhejiang Hisun Pharmaceutical Co., Ltd.
- Publication Type:Journal Article
- Keywords:
Biosynthesis;
HPLC;
Lovastatin J;
Monacolin J;
Simvastatin
- From:
Chinese Pharmaceutical Journal
2012;47(7):543-546
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish an HPLC analysis method of monacolin J(MJ). METHODS: MJ sample was analyzed with Hypersil C18 HPLC column. The mobile phase was composed of 0.1% H3PO4 and acetonitrile (30:70) with flow rate of 1.0 mL · min-1, and the detection wavelength was 238 nm. Monacolin J samples produced from lovastatin fermentation mycelia and engineered Aspergilus terreus were analyzed. RESULTS: The calibration curve of monacolin J was linear in the range of 10 - 500 μg · mL-1 with r of 0.9998. The purity of crystaline monacolin J from lovastatin fermentation mycelia was 92%, and the purification yield was 96.24%. Meanwhile the engineered Aspergilus terreus produced lovastatin and monacolin J at the ratio of about 1.1:1.0. CONCLUSION: This HPLC method can separate lactone form and acid form of monacolin J, lovastatin and simvastatin very well. This HPLC method can be effectively used in monacolin J research of simvastatin biosynthesis. Copyright 2012 by the Chinese Pharmaceutical Association.
