Pharmacokinetic study of total glucosides of paeony in rats with acute liver injury and normal rats
- Author:
En-Li LIU
1
Author Information
1. School of Pharmaceutical Science
- Publication Type:Journal Article
- Keywords:
Acute liver injury rat model;
Albiflorin;
Paeoniflorin;
Pharmacokinetics;
Total glucosides of paeony
- From:
Chinese Pharmaceutical Journal
2012;47(7):534-538
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To compare the pharmacokinetics of total glucosides of paeony (TGP) in rats with acute liver injury and normal rats, thus to provide evidence for determining dosage regimen in clinic. METHODS: Acute liver injury rat model was established by single CCl4 intraperitoneal injection. Three dosages of TGP were orally administered to normal and model rats. The concentrations of Pae and Alb in plasma at different time after dosing were determined with HPLC method. The pharmacokinetic parameters were calculated based on mean plasma concentration-time curves of Pae and Alb. RESULTS: In model rat group, the t1/2 of Pae and Alb at high, medium, and low dosage (0.047, 0.141, 0.282 g · mL-1) were 5.02, 4.52, 4.91 h and 5.22, 5.04, 5.05 h, respectively. The ρmax of Pae and Alb of TGP at different dosages were 22.54, 14.52, 5.49 μg · mL-1, and 9.02, 5.04, 2.34 μg · mL-1, respectively. The AUC0-t of Pae and Alb of TGP at different dosages were (146.55 ± 7.52), (84.14 ± 7.84), (31.62 ± 2.97) μg · h · mL-1, and (56.56 ±8.70), (32.18 ± 3.49), (13.48 ±1.66) μg · h · mL-1, respectively. The tmax of paeoniflorin(Pae) and albiflorin(Alb) of TGP at different dosages were both 2 h. In rats of normal group, the t1/2 of Pae and Alb of TGP at different dosages respectively were 3.95, 3.69, 3.95 h, and 3.74, 3.98, 3.79 h, respectively. The ρmax were 19.16, 11.60, 4.46 μg · mL-1, and 7.16, 4.00, 1.93 μg · mL-1, respectively. The AUC0-t were (116.26.6 ± 19.99), (67.74 ± 12.79), (25.01 ±2.62) μg · h · mL-1, and (45.03 ±5.84), (27.26 ±3.57), (10.59 ± 1.86) μg · h · mL-1, respectively. The tmax were both 2.5 h. CONCLUSION: Compared with normal group, the ρmax and AUC0-t of TGP in model group significantly increased, tmax decreased, and t1/2 prolonged; tmax and t1/2 had no difference among three dosages, and ρmax. and AUC0-t had correlation with dosage. Copyright 2012 by the Chinese Pharmaceutical Association.
