Simultaneous determination of five index components in Eucommiae Cortex by HPCE
- Author:
Xing-Sheng FU
1
Author Information
1. Jiangsu Key Laboratory of Chinese Medicines Processing
- Publication Type:Journal Article
- Keywords:
Aucubin;
Chlorogenic acid;
Eucommiae Cortex;
Geniposide;
Harpagide;
HPCE;
Iridoid glycoside;
Pinoresinol diglucoside
- From:
Chinese Pharmaceutical Journal
2012;47(9):720-723
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a high performance capillary electrophoresis (HPCE) method for simultaneous determination of aucubin, geniposide, pinoresinol diglucoside, harpagide, and chlorogenic acid in Eucommiae Cortex. METHODS: 60 mmol·L-1 sodium borate-20 mmol·L-1 sodium dihydrogen phosphate-10% methanol was used as buffer solution (pH 10.0), un-coated fused silica capillary (64.5 cm×75 μm id, 56 cm of effective length) was used, separation voltage was 20 kV, detection wavelength was 210 nm, column temperature was maintained at 25°C, and sample was injected at 5 kPa×6 s. RESULTS: Five index components showed good linearity (r>0.9973) in the range of the tested concentration. The average recoveries of the method were between 96.63%-103.73%. CONCLUSION: The method is simple, accurate and reproducible, and can be used for quality control of Eucommiae Cortex. Copyright 2012 by the Chinese Pharmaceutical Association.
