Inhibitory effects and mechanism of extracts from Guizhi Fuling on the proliferation in human cervical carcinoma cells
- Author:
Lian-Hua FANG
1
Author Information
1. State Key Laboratory for Bioactive Substances and Functions of Natural Medicines
- Publication Type:Journal Article
- Keywords:
Anticarcinoma;
Antioxidation;
Cervical carcinoma;
Extracts;
Guizhi Fuling;
Protein tyrosine kinases
- From:
Chinese Pharmaceutical Journal
2012;47(10):813-817
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To investigate the inhibitory effects of different solvent extracts from Guizhi Fuling on the proliferation in human cervical carcinoma cells and its possible mechanisms. METHODS: MTT assay was used to detect the proliferation in the HeLa and C33A human cervical carcinoma cells, and the enzyme-linked immunosorbent assay was used detect for the protein tyrosine kinases (PTKs) activity in vitro, and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging ratio displayed antioxidant activity of different extracts from Guizhi Fuling. RESULTS: Most of different solvent extracts from Guizhi Fuling, including extracts of ethyl acetate, ethyl acetate-water, ethyl acetate-acetone, butyl alcohol-30% ethanol and butyl alcohol-50% ethanol, displayed good inhibitory activity on the proliferation in human cervical carcinoma cells. The inhibit potency to the largest for HeLa cells was butyl alcohol-30% ethanol eluate, its IC50 was (7.09±1.25) μg·mL-1, and inhibit potency to the largest for C33A cells was ethyl acetate-acetone eluate, its IC50 was (5.14±0.70) μg·mL-1. CONCLUSION: These results suggested that different solvent extracts from Guizhi Fuling, have different anticancer activity in vitro. Its mechanism may be associated with inhibition of PTKs and antioxidation activity. Copyright 2012 by the Chinese Pharmaceutical Association.
