Separation and purification of naphthaquinone components from rhizoma of Arnebia euchroma(Royle) johnst by medium and low pressure preparative chromatography
- Author:
Jun-Fei GAO
1
Author Information
1. Wuhan Botanical Garden
- Publication Type:Journal Article
- Keywords:
β,β -dimethylacrylshikonin;
β-acetoxyisovalerylshikonin;
Acetylshikonin;
Deoxyshikonin;
Isobutylshikonin;
Medium and low pressure preparative chromatography;
Shikonin
- From:
Chinese Pharmaceutical Journal
2012;47(11):923-926
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a new chromatography technique to, separate and purify naphthaquinone components from rhizoma of Arnebia euchroma(Royle). METHODS: Medium and low pressure preparative chromatography and Flash column were used to separate and purify the constituents, and their structures were elucidated by physicochemical properties, IR, MS, 1H-NMR and C-NMR. RESULTS: Six monocases were obtained, which were deoxyshikonin, shikonin, isobutylshikonin, acetylshikonin, β-ace-toxyisovalerylshikonin, and β, β-dimethylacrylshikonin. The purities of the 6 compounds analyzed by HPLC were 99.62%, 99.02%, 99.5%, 99.31%, 99.3% and 99.2%, respectively. CONCLUSION: A medium and low pressure preparative chromatography method has been established for the first time which can accurately and rapidly separate monocases from herbs.
