Study on intestinal absorption mechanism and kinetics of dauricine in rats with in situ single-pass perfusion model
- Author:
Xiu-Rong GAO
1
Author Information
1. The Department of Pharmacy
- Publication Type:Journal Article
- Keywords:
Absorption dynamics;
Absorption mechanism;
Dauricine;
HPLC;
In situ single-pass perfusion model
- From:
Chinese Pharmaceutical Journal
2012;47(11):903-907
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To investigate the absorption characteristics of dauricine in rat intestine. METHODS: In situ single-pass perfusion model was used and the concentrations of dauricine in perfusate were determined by HPLC. The effects of perfusion rates, intestinal segments and drug concentrations on the intestinal absorption of dauricine were studied. RESULTS: The absorption rate and absorption degree of dauricine increased with the perfusion rates(0.1, 0.2 and 0.4 mL · min-1)(P<0.05); the absorption rate constants of dauricine in duodenum, jejunum, ileum and colon were not significantly different(P>0.05); at high, middle and low concentrations, the drug absorption rate constants(Ka) were (2.36±0.0073) × 10-2, (3.73 ± 0.0052) × 10-2 and(5.62 ± 0.0136) × 10-2 min-1, respectively, the apparent permeation coefficients(P.,) were(2.02±0.0002) × 103, (3.10±0.0007) × 10-3 and (5.31±0.0010) × 10-3 cm · min-1, respectively, the absorption percentages(P%) were 8.66%, 10.17% and 19.06%, respectively, and the accumulate absorption amount and accumulate absorption percentages of different concentrations at different time were very low. CONCLUSION: The absorption degree of dauricine increases with perfusion rates; there is no specific absorption site in the whole rat intestinal tract; the absorption of dauricine is very poor and the active transport is involved in the absorption mechanism of dauricine.
