Determination of cinnamic acid, cinnama ldehyde, paeonol, alisol B 23-acetate, atractylodes lancea and atractylodin in Yeming granules by RP-HPLC
- Author:
Hui-Min YAO
1
Author Information
1. Department of Pharmacy and Food Engineering
- Publication Type:Journal Article
- Keywords:
Alisol B 23-acetate;
Atractylodes lancea;
Atractylodin;
Cinnama ldehyde;
Cinnamic acid;
Paeonol;
RP-HPLC;
Yeming granules
- From:
Chinese Pharmaceutical Journal
2012;47(15):1242-1245
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To develop a RP-HPLC method for determination of cinnamic acid, cinnama ldehyde, paeonol, alisol B 23 acetate, atractylodes lancea, and atractylodin in Yeming granules. METHODS: The determination was performed on an Eclipse XDB-C18column (4.6 mm × 250 mm, 5 μm) at 30°C with a flow rate of 1.0 mL · min-1. The mobile phase was 0.3% phosphate-acetonitrile at gradient elution. The detection wavelength was set at 240 nm. RESULTS: The linear regression equations for cinnamic acid, cinnama ldehyde, paeonol, alisolB 23 acetate, atractylodes lancea, and atractylodin were ρ=-0.0055A + 0.0083 (r=0.9996), ρ=0.0255, 4+0.1336 (r=0.9999), ρ=0.0408 A +0.664 3 (r=0.999), ρ=0.003 3 4+0.0171(r=0.999 9), ρ=0.0163 A + 0.1075 (r=0.999 8), ρ=0.0229A + 0.0884 (r=0.9999), respectively. Good linearity was obtained over the ranges of 0.3096-2.322, 10.24-76.8, 37.36-280.2, 0.5128-3.846, 1.612-12.09 and 10.26-76.95 μg · mL-1, respectively. The recoveries were 100.1%, 99.8%, 100.7%, 99.3%, 100.4% and 100.5%, respectively. CONCLUSION: The method is simple and suitable to control the quality of Yeming granules. Copyright 2012 by the Chinese Pharmaceutical Association.
