Preparation and in vivo irritating study of doxycycline hyclate in situ forming sustained release injection
- Author:
Mei-Yan YANG
1
Author Information
1. Institute of Pharmacology and Toxicology
- Publication Type:Journal Article
- Keywords:
Doxycycline hyclate;
In situ forming gel;
Injectable;
Periodontal disease;
PLGA;
Sustained release
- From:
Chinese Pharmaceutical Journal
2012;47(16):1313-1317
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To optimize the formulation and study the irritation of doxycycline hyclate in situ forming sustained release injection. METHODS: Doxycycline in situ forming gel (DHISFG) was prepared by using biodegradable, biocompatible PLGA and PLA as materials, and non-toxic NMP as solvent. Orthogonal design was used to optimize the gel formulation. The in vitro drug release, rheology and irritation were also studied. RESULTS: The optimal formulation of DHISFG was PLGA (Mn=10000)-NMP-doxycycline hyclate=25:65:10. The drug release from the DHISFG was 20% within 2 hours and above 90% after 10 days sustained release. The in vitro release data fit the first release and Fick diffusion mechanism. Gingival irritation test of rabbit showed no irritation. CONCLUSION: The developed DHISFG which is degradable and easy to inject could provide a new sustained release formulation to relieve the symptoms of periodontal disease such as alveolar bone absorption and tooth loss. Copyright 2012 by the Chinese Pharmaceutical Association.
